qPCR/real-time-PCR Master Mix DLP1

qPCR/RTD-PCR Master mix with dUTP (for dual-labeled probes)

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix contains dUTP instead of dTTP and allows an UNG (Uracil-N-Glycosylase) treatment at the onset of thermal cycling to prevent carry-over contaminations of DNA from previous PCR reactions.

Features:
– optimized realtime PCR Mastermix using probe based detection (e.g.
FRET, Molecular Beacons or TaqMan)
– The Master mix contains dUTP instead of dTTP
– The qPCR / RT-PCR Mastermix DLP1 is ready-to-use and is optimized
for high specificity and sensitivity because of optimized reaction buffer
– easy to use because ready-to-use Master Mix for block based PCR
Cycler
– The Master Mix can be used with ROX as reference dye (1x
concentrated)

Applications:
– Detection and quantification of DNA and cDNA targets
– Profiling gene expression
– Microbial detection
– Viral load determination

Concentration: The Master mix is 2x concentrated

List of components:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, optimized reaction buffer with KCl and MgCl₂, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000