Enhancer for Polymerase reactions at roomtemperature / monoclonal antibody to Taq DNA Polymerase
Features:
– Anti–Taq (monoclonal antibody to Taq DNA polymerase) inhibits the
reaction of Taq DNA Polymerases at room temperature
– Ideal as enhancer for PCR reactions or for “homemade” Hot-Start
Polymerase
Application:
Polymerase detergent/enhancer for:
– High specific amplification
– Multiplex amplification
– High sensitivity applications
– Low-copy number PCR
Unit definition:
One unit is defined as the amount required to blocks 50% activity of 1 µg of Polymerase
Note: The ratio units/mg of polymerase varies (up to factor of 10). The binding rate Anti–Taq depends strongly of the polymerization region (active epitopes) of the polymerase. The optimized mixture has to be found in empiric test and for every new lot of polymerase, again.
Storage Buffer for S131/S132
20mM Tris-HCl (pH 7.0, at 22oC);50 mM KCl;0.1mM EDTA; 50% glycerol
Storage Buffer for S131/S132-8C1
3mM Tris-HCl (pH 8,0, @ 22oC); 15 mM KCl; 0.1mM EDTA; 20 % Trehalose (pH 8,0, @ 22oC);
Concentration: 2-10 mg/ml
Units/mg-ratio:
2300 units of the specific polymerase activity are equal to 1 mg of antibodies
Storage: at – 20°C
Transportation: at room temperature or with blue ice
Stability tests / Quality control / Comparison
QC-Test: > 95% of protein in SDS electrophoresis in 15% PAAG
| Product | Description |
| S9131 |
m-Anti Taq – monoclonal antibody to Taq DNA Polymerase 100 µg |
| S9132 |
m-Anti Taq – monoclonal antibody to Taq DNA Polymerase 500 µg |
| S9131-8C1 |
m-Anti Taq – monoclonal antibody to Taq DNA Polymerase buffered in Trehalose 100 µg |
| S9132-8C1 |
m-Anti Taq – monoclonal antibody to Taq DNA Polymerase buffered in Trehalose 100 µg |
How to mix m-Antitaq with Taq DNA Polymerase?
| Name | Final conc., M |
| Tris-HCl, pH 8,5 | 0,05-0,1 |
| KCl | 0,05-0,1 |
| MgCl2 | 0,01-0,0015 |
| TMAC* | 0,015-0,034 |
| Glycerol, % | from 3 to 6 |
| BSA, mg/ml | 0,1-0,2 |
| Tween 20, % | 0,15-0,2 |
| Taq polymerase, U/microliter | 0,05-0,1 |
| Trehalose MABs, mg/ml | 0,0036-0,0072 |
*TMAC – Tetramethylammonium chloride
IMPORTANT: The proportion DFS PLUS Taq / m-AntiTaq should be 1 Unit to 0,06-0,072 µg MAB
Datasheet
Stability Test Report
Material Safety Datasheet
References
1.) R.T. D’Aquila, L.J. Bechtel, J.A. Videler, Eron JJ, P. Gorczyca, J.C.Kaplan, Maximizing sensitivity and specificity of PCR by pre-amplification heating. Nucleic Acids Res. 19: 3749 (1991)
2.) Q. Chou, M. Russell, D.E. Birch, J. Raymond, W. Bloch. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20: 1717-23 (1992)
3.) D.E. Kellogg , I. Rybalkin, S. Chen, N. Mukhamedova, T. Vlasik, P.D. Siebert, A. Chenchik. TaqStart Antibody: “hot start” PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques. 16: 1134-7 (1994)