Maximo Taq Polymerase is a highly purified polymerase for routine amplification
Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.
Applications:
– Standard / General PCR
– Multiplex PCR
– High-throughput PCR
– Primer extension
– Gene mutation
– T/A cloning
Description:
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
Concentration: 5 u/µl
Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree
Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20
Reaction Buffers supplied with the enzyme:
10X Buffer I: 500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl2
Quality control:
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 3 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination
Transportation: on blue ice
Storage: at -20°C for 24 months
| Product | Description |
| S9101 |
Maximo Taq DNA Polymerase 500 units |
| S9102 |
Maximo Taq DNA Polymerase 5 x 500 units |
| S9103 |
Maximo Taq DNA Polymerase 20 x 500 units |
Usage:
| Components | Volume per reaction |
| 10X reaction buffer I or buffer II | 5 µl |
| 25 mM MgCl2 | 1.5 µl (if you use buffer II) |
| dNTP-Mix (40mM) | 1.0 µl |
| Up-stream primer (10 µM stock) | 0,5-2.5 µl |
| Down-stream primer (10µM stock) | 0.5-2,5 µl |
| Template DNA | 0.1-15 ng/ml plasmid DNA 1-10 µg/ml genomic DNA |
| Maximo Taq DNA Polymerase (5 u/µl) | 0.2 – 1.0 µl |
| Sterile dest. Water (molecular grade) | up to 50 µl total reaction volume |
Note:
– vortex all solutions carefully before using
– dispense all reagents on ice
– add the enzyme after Template DNA
– may you have to optimize the MgCl2 concentration for best result
General Thermo-Cycler protocol:
| Step | Time | Temperature |
| Initial denaturation | 2-5 min | 94-95°C |
|
25-30 Cycles: |
||
|
Denaturation Annealing Extension |
10-25 sec 10-25 sec 60 sec |
94-95°C 55-65°C 72°C per 1kb |
| Final extension | 5 min | 72°C |
Datasheet
Stability Test Report
Material Safety Datasheet
