Ready-to-use lyophilized PCR-Master Mix in several Reaction chambers like tubes or PCR-Plates
Features:
– All components like: Taq polymerase, dNTP’s, reaction buffers,
enhancers and stabilizers are lyophilized
– Transportation and at room-temperature
– simple, and fast setting up procedure for high yield and repeatable
results
Description:
Maximo Dry Master-Mix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template has to be added to the 20 µl assay.
Maximo Dry Master-Mix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
List of components:
12×8 lyophilized PCR-Tubes (0,2ml) “flat cap”,
Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74°C
Quality control:
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 2 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination
Transportation: at room temperature
Storage: store at room-temperature in an aluminium coated bag or on another dry place; humidity < 65 % when sealing is opended
Quality control:
– PCR with various templates – genomic DNA, Phage Lambda DNA
– 2 kb DNA amplification from 50 ng DNA
– batch variation and level of bacterial DNA contamination
| Product | Description |
| S9295 |
Maximo Dry-Master Mix (20µl) 12×8 Flat-Cap PCR Tubes |
Usage:
| Components | Volume per reaction |
| 1 Tube of Maximo Dry Mastermix | |
| Forward Primer: | 0,2-1 µM – 0,4-2µl / 10 µM |
| Reverse Primer | 0,2-1 µM – 0,4-2µl / 10 µM |
| Template DNA | 1-50 ng |
| Sterile dest. Water (molecular grade) | up to 20 µl total reaction volume |
Note:
– vortex all solutions and spin down carefully before using
Datasheet
Stability Test Report
Material Safety Datasheet
