100bp Plus DNA Ladder (no dye)

Features: GeneON 100 bp DNA Plus Marker is composed of 11 individual DNA fragments which are re-dissolved in storage buffer.

Description/Preparation: The 100 bp PLUS DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis.  The 100 bp DNA Ladder was designed for precise qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 500bp fragment is used a reference band.

Usage: 1 µg/lane

Concentration:  0,2 µg/µl

Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl

Tracking Dye: 6X DNA Loading dye required

Gel-picture: 1 μg of 100bp + 1,5Kb DNA Ladder visualized by ethidium bromide staining on 1,7 % TAE agarose gel

Number of bands: 11 fragments

Number of bands and approx. DNA Mass:

Loading: 0.5 – max. 1.2 µg/ lane in 1.7 – 2.5 % Agarose. We recommend to add the loading dye in amount 1/10 from the DNA Ladder volume

Transportation: shipped on blue ice or room temperature

Storage: at -20°C for 24 months